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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human herpesvirus 2 Protein UL20 (UL20), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human herpesvirus 2 Protein UL20 (UL20), partial品名简称:
Recombinant Human herpesvirus 2 UL20 protein, partial货号:
CSB-EP310461HJX1规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
UL20Uniprot No.:
P89443别名:
/种属:
Human herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)蛋白长度:
Partial来源:
E.coli分子量:
14 kDa表达区域:
1-63aa氨基酸序列:
MTMRDDVPLLDRELVDEAACGGEDGELPLDEQFSLSSYGTSDFFVSSAYSRLPPHTQPVFSKR蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Plays an essential role in egress of virus particles from the nucleus, cytoplasmic envelopment and virus-induced cell fusion. Forms a functional protein complex with gK and this interaction is absolutely essential for their coordinate intracellular transport, gK glycosylation, expression on host cell surface, and function. Together, they modulate gB-mediated virus-induced cell fusion and virion egress and therefore actively participate in these processes.功能2:
/文献:
/研究领域:
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文献和实验Acetone precipitation of protein
to the tube. 4. Vortex tube and incubate for 2 hours to overnight minutes at -20°C. 5. Centrifuge 15 minutes at 13,000-15,000 x g at 4°C. 6. Decant and properly dispose of the supernatant, being careful to not dislodge the protein pellet.
Preparing a Selenomethionyl Protein
filtered) 2M CaCl2 (sterile filtered) 0.5% (w/v) Thiamine Solution (sterile filtered) Procedure Day 1 Prepare a 2mL day culture consisting of 2mL LB media, 2uL antibiotics (1000x conc.), and a single E.coli colony. Grow at 37
Protein extraction from whole tissues for IEF
of methanol 80% acetone (100 ml) - store at -20 C 70% ethanol (100 ml) - store at -20 C IEF Sample Buffer (10 ml) 8 M urea 4.85 g 2 M thiourea 1.52 g 2% CHAPS 0.2 g 2% Triton X-100 200 ul 50 mM DTT 8 mg/ml make to 10 ml
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