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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Probable phospholipid-binding lipoprotein mlaA (mlaA)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Probable phospholipid-binding lipoprotein mlaA (mlaA)品名简称:
Recombinant E.coli mlaA protein货号:
CSB-EP300563ENV规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
mlaAUniprot No.:
P76506别名:
/种属:
Escherichia coli (strain K12)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
30.4 kDa表达区域:
18-251aa氨基酸序列:
CASSGTDQQGRSDPLEGFNRTMYNFNFNVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRDDGGDMADGFYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGELKPQENPNAQAIQDDLKDIDSE蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Involved in a phospholipid transport pathway that maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane.功能2:
/文献:
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006)研究领域:
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文献和实验。 细菌来源细胞因子 上世纪70年代出现的基因工程重组技术,使得使用原核细菌宿主,如大肠杆菌进行天然蛋白的重组表达和生产风靡一时,因其速度快、生产成本低、产率高等优点,广泛用于包括细胞因子在内的多种重组蛋白表达的科研和生物制药领域。但随之而来,使用低等细菌进行蛋白表达的技术弊端也日益显现:低等原核细菌宿主表达的细胞因子,其结构和天然细胞因子蛋白有较大的差异。由于缺乏高等动物细胞中特有的各种细胞器,细菌表达的蛋白肽链无法完成翻译后修饰,缺乏必要的糖基化和磷酸化,二硫键配对完全随机;细菌
in recombinant Escherichia coli, Biochem. J. 358 (2001) 263-268. 35. G. Lemercier, N. Bakalara, X. Santarelli, On-column refolding of an insoluble histidine tag recombinant exopolyphosphatase from Trypanosoma brucei overexpressed in Escherichia coli, J
摘要 基因重组蛋白在大肠杆菌中表达时,由于表达量高,往往形成无生物活性的包涵体。包涵体必须经过变性和复性的过程才能获得有活性的重组蛋白。如何提高基因重组蛋白质的复性率,是生物工程技术的一个研究热点。对近年来的重组蛋白质的复性方法做一评述,为研究蛋白质折叠以及复性技术的进一步应用提供依据。 关键词 重组蛋白 包涵体 复性 二硫键 到目前为止,人们表达的重组蛋白质已有4000多种,其中用E.coli表达的蛋白质要占90%以上,尽管基因重组技术为大规模生产目标蛋白质提供
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