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文献和实验Synthesis of Double-Stranded Complementary DNA from Poly(A)+mRNA
from a short double-stranded region, usually provided by using an oligo(dT) primer on poly(A) + RNA. The enzyme does not always produce full length transcripts, but all the complementary strands are finished off with a short hairpin loop. This provides a ready
Methods for Delivery of Double-Stranded RNA into Caenorhabditis elegans
The nematode Caenorhabditis elegans is often employed in investigations of diverse aspects of biology, including behavior, development, basic cellular processes, and disease states. The ability to utilize double-stranded RNA (dsRNA
Gene Knockdown Analysis by Double-Stranded RNA Injection
The discovery of RNAi, in which double-stranded RNA (dsRNA) suppresses the translation of homologous mRNA, has had a huge impact on evolutionary genetics by enabling the analysis of loss-of-function phenotypes in organisms in which classical
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