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文献和实验The K/BxN Mouse Model of Inflammatory Arthritis: Theory and Practice
Mice expressing the KRN T cell receptor transgene and the MHC class II molecule Ag7 (K/B�N mice) develop severe inflammatory arthritis, and serum from these mice causes similar arthritis in a wide range of mouse strains, owing to pathogenic
Non-fluorescent protein/RNA double-labeling
) at room temperature. 2. Wash 3 X 5 minutes with PBT. 3. Incubate the embryos with 50 ug/ml of Proteinase K in PBT for 2-3 minutes (time varies with different Prot. K preps and needs to be optimized). 4. Stop the Proteinase K reaction
Non-fluorescent protein/RNA double-labeling
Non-fluorescent protein/RNA double-labeling (a version of this is described in: Manoukian, A. and Krause, H.M.(1992) Genes Dev ., 6: 1740-1751.) 1. Collect embryos, dechorionate and fix in a (1:1) mixture of heptane:fix. Fix
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