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- 湖北武汉
- ABL1020
- 2025年11月17日
- WB#IHC-P#IF
- 鼠
- 人#小鼠#大鼠#猴#狗#鸡#兔#猪#绵羊#酵母#仓鼠
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
合成多肽
- 亚型：
鼠源IgG
- 形态：
液体形式
- 保存条件：
自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
- 克隆性：
单克隆
- 标记物：
Null
- 适应物种：
人#小鼠#大鼠#猴#狗#鸡#兔#猪#绵羊#酵母#仓鼠
- 保质期：
Null
- 抗原来源：
鼠
- 目录编号：
Null
- 级别：
Null
- 库存：
现货
- 供应商：
亚科因(Abbkine)
- 宿主：
鼠
- 应用范围：
WB#IHC-P#IF
- 浓度：
1 mg/ml
- 靶点：
Anti-GAPDH Mouse Monoclonal Antibody (2B5)
- 抗体英文名：
Anti-GAPDH Mouse Monoclonal Antibody (2B5)
- 抗体名：
GAPDH鼠单克隆抗体
- 规格：
50 μL/200 μL/200 μL×5
| 规格： | 50 μL | 产品价格： | ￥498.0 |
|---|---|---|---|
| 规格： | 200 μL | 产品价格： | ￥898.0 |
| 规格： | 200 μL×5 | 产品价格： | ￥3998.0 |
Anti-GAPDH Mouse Monoclonal Antibody (2B5)
产品名称： GAPDH鼠单克隆抗体
货号： ABL1020
产品类型： 内参/标签抗体
一级应用： 核心产品
产品分类： 样品制备及检测
产品概述
该抗体针对甘油醛-3-磷酸脱氢酶(GAPDH)，是一种鼠源单克隆抗体，适用于Western Blot (WB)、免疫组化(IHC-P)和免疫荧光(IF)等检测应用。它设计用于研究GAPDH蛋白在多种模型中的表达分析。
背景： GAPDH（基因ID: 2597）是一种37kDa酶，催化糖酵解第六步，并涉及转录激活、细胞凋亡等非代谢过程。
关键特性
- 宿主： 鼠
- 反应性： 人、小鼠、大鼠、猴、狗、鸡、兔、猪、绵羊、酵母、仓鼠
- 检测类型： WB, IHC-P, IF
- 克隆类型： 单克隆
- 亚型： 鼠源IgG
- 纯化工艺： 通过使用特异性免疫原的亲和层析纯化抗体
- 免疫原： 合成多肽
- 产品形式： 液体
- 浓度： 1 mg/ml
- 目标条带分子量： 37 kDa
- 蛋白质ID： P04406
- 别名： GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH
- 其他： 该抗体可检测内源蛋白
储存与处理
储存缓冲液： PBS缓冲液（pH 7.4），含有0.02% NaN₃防腐剂和 50% 甘油。
保存建议： 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。建议使用前分装以避免反复冻融。
运输条件： 冰袋运输（蓝冰）
规格与价格
| 规格 | 价格 (¥) | 货期 |
|---|---|---|
| 50 μL | 498 | 现货（当天发货） |
| 200 μL | 898 | 现货（当天发货） |
| 200 μL × 5 | 3998 | 期货（3天内发货） |
警告
本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。
Anti-GAPDH Mouse Monoclonal Antibody (2B5)
Product Name: GAPDH Mouse Monoclonal Antibody
Catalog Number: ABL1020
Product Type: Loading Control/Tag Antibody
Primary Application: Core Product
Product Category: Sample Preparation & Detection
Overview
This monoclonal antibody targets Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), designed for expression analysis in Western Blot (WB), Immunohistochemistry (IHC-P), and Immunofluorescence (IF) applications across multiple research models.
Background: GAPDH (Gene ID: 2597) is a 37kDa enzyme catalyzing the sixth step of glycolysis, with recently identified roles in non-metabolic processes including transcriptional activation and apoptosis.
Key Specifications
- Host: Mouse
- Reactivity: Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Yeast, Hamster
- Applications: WB, IHC-P, IF
- Clonality: Monoclonal
- Isotype: Mouse IgG
- Purification: Affinity purified using immunogen-specific chromatography
- Immunogen: Synthetic peptide
- Form: Liquid
- Concentration: 1 mg/ml
- Target Band Size: 37 kDa
- UniProt ID: P04406
- Aliases: GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH
- Note: Detects endogenous protein
Storage & Handling
Buffer: PBS (pH 7.4) containing 0.02% sodium azide and 50% glycerol.
Storage: Stable for 1 year at -20°C from date of shipment. Thaw and centrifuge prior to opening. Aliquot recommended to avoid repeated freeze/thaw cycles.
Shipping: Blue ice packs
Packaging & Pricing
| Size | Price (¥) | Lead Time |
|---|---|---|
| 50 μL | 498 | In Stock (Same Day Shipment) |
| 200 μL | 898 | In Stock (Same Day Shipment) |
| 200 μL × 5 | 3998 | 3 Days Production |
Warning
This product is for research use only. Not intended for diagnostic or therapeutic procedures. Use of this product does not imply license under any patents. We assume no liability for patent infringement or regulatory non-compliance arising from product use.
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，但二者均未扩出；.......提示目的基因及内参可能有问题？用肯定可以扩出内参的RT产物包括人，鼠样本各一，以验证是否内参存在问题，结果人cDNA可扩出内参GAPDH，鼠cDNA未见内参GAPDH；......提示内参只能扩人，而我的样本为鼠，所以出现目的基因及内参均未扩出的结果，故不能就此推论RT有误。为进一步明确是否内参引物同时有问题，再次用GAPDH扩鼠肯定可以扩出内参的cDNA，仍无结果；.......证实GAPDH不能起到内参作用。那么问题在哪呢？？？？？？？RT过程RNA降解？（操作中
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