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文献和实验实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
Isolation of Mouse Thymic Dendritic Cell Precursors
+ Sirpαl � cDC are generated in the thymus by the earliest intrathymic oligo-potent progenitors that are also precursors for T-lineage cells and natural killer cells (NK cells). Whereas the CD8αl �Sirpα+ cDC and pDC are migratory DC and originate mainly
Lentiviral Gene Transduction in Human and Mouse NK Cell Lines
cells. We have standardized methods of lentiviral infection in human and mouse NK cell lines. We obtain transduction efficiencies of 15% in the NK-92 cell line and 30–40% in LNK, YT, and DERL7 cell lines. This method allows efficient and stable
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