APC Annexin V Apoptosis Detect

Annexin V 凋亡试剂盒常见问题

择？ A：第一步考虑流式细胞仪配置，第二步考虑细胞自发荧光。对于本身有荧光的细胞（如转染了荧光蛋白基因的细胞或者药物孵育的细胞），特别是培养时间久或药物处理的细胞，一定要注意细胞代谢物和药物的荧光。一般来说，细胞代谢物的荧光会干扰 FITC/PE，所以建议用 Annexin V-APC，药物的干扰可查看药物本身的发射光谱是否会对检测选择的染料有影响。 Annexin V-APC 与 7-AAD 两种染料之间补偿干扰很小，染色操作简单，实验准确。 7-AAD 染料相比于 PI 染料粘稠度小，可减少检测

Annexin V detection protocol

protocol - Protocol for Annexin V-FITC Apoptosis Detection Kit (ab14085): 1. Incubation of cells with Annexin V-FITC     1) Induce apoptosis by desired method.     2) Collect 1-5 x 105 cells by centrifugation.     3) Resuspend cells

Apoptosis Measurement by Annexin V Staining

is associated with the condensed chromatin in apoptotic cells as a measure of apoptosis. It may appear as early as 3 h post the apoptosis inducing event and precedes the ability to detect actual DNA fragmentation. The annexin V assay has been widely accepted