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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD
2 (2a/2b), IgG3, IgG4 IgA：IgA1, IgA2 IgM: IgM1, IgM2 IgD/ IgE : not found 2.型和亚型(根据轻链C区抗原特异性不同分型) 型(type): κ(kappa)型 (human 60%/mouse 95%)；λ(lambda)型 (human 40%/mouse 5%) 同一天然Ig分子中两条轻链的型必然相同/同一个体内可有不同型的Ig亚型(λ链)(subtype
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