
Hoechst 33342
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- 保存条件:
-20℃,避光保存12个月。
- 保质期:
-20℃,避光保存12个月。
- 英文名:
Hoechst 33342
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 规格:
10 mg/100 mg
| 规格: | 10 mg | 产品价格: | ¥298.0 |
|---|---|---|---|
| 规格: | 100 mg | 产品价格: | ¥2206.0 |
Hoechst 33342(货号:BMD0062)
分子式:C27H28N6O•3HCl•3H2O | 分子量:615.99 kDa
产品概述
本品为黄色固体,专为细胞器提取、染色及核复染色设计,适用于细胞周期、凋亡研究等细胞分析领域。
特点与优势
- 荧光特性:λEx/λEm=350/461 nm(结合DNA),346/460 nm(未结合DNA)
- 非嵌入性染料:亮蓝色荧光标记,减少对DNA结构干扰
应用背景
Hoechst 33342作为经典核染色剂,可穿透完整细胞膜,广泛应用于活细胞/固定细胞染色、多色荧光成像及高通量筛选。
保存与运输
- 保存条件:-20℃避光保存,有效期12个月
- 运输方式:冰袋运输(蓝冰)
注意事项
- 水溶性配方,建议用超纯水配制储存液
- 避免反复冻融,推荐分装保存
产品规格
现货:10 mg | ¥298 | 当天发货
期货:100 mg | ¥2206 | 4个工作日内发货
安全声明
警告:本品仅限研究用途,不可用于人类或临床诊断。使用前请确认不违反当地法规及知识产权要求。
搜索关键词:Hoechst 33342 ; Hoechst ; BMD00062
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注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Hoechst 33342 (Cat No.: BMD0062)
Molecular Formula: C27H28N6O•3HCl•3H2O | Molecular Weight: 615.99 kDa
Product Overview
This product is a yellow solid, specifically designed for organelle extraction, staining, and nuclear counterstaining, suitable for cellular analysis fields such as cell cycle and apoptosis research.
Features & Advantages
- Fluorescence Properties: λEx/λEm=350/461 nm (DNA-bound), 346/460 nm (unbound DNA)
- Non-intercalating Dye: Bright blue fluorescence labeling with minimal interference to DNA structure
Application Background
As a classic nuclear stain, Hoechst 33342 can penetrate intact cell membranes and is widely used for live/fixed cell staining, multicolor fluorescence imaging, and high-throughput screening.
Storage & Shipping
- Storage Conditions: Store at -20°C protected from light; valid for 12 months
- Shipping Method: Ice pack shipment (blue ice)
Precautions
- Water-soluble formulation; prepare stock solution using ultrapure water
- Avoid repeated freeze-thaw cycles; aliquot storage is recommended
Specifications
In Stock: 10 mg | ¥298 | Same-day shipping
Pre-order: 100 mg | ¥2206 | Ships within 4 business days
Safety Statement
Warning: This product is for research use only, not for human or clinical diagnostics. Ensure compliance with local regulations and intellectual property requirements before use.
Search Keywords: Hoechst 33342 ; Hoechst ; BMD00062
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Note: The above information may be subject to changes due to product optimization and upgrades. For specific details, please consult Yakeyin Company.
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文献和实验Comparative cytotoxicity, endocrine-disrupting effects, oxidative stress of halophenolic disinfection byproducts and the underlying molecular mechanisms revealed by …
作者:Li, Xiuwen, et al. 杂志名称:Water Research IF:4
Isolation of Resident Cardiac Progenitor Cells by Hoechst 33342 Staining
of stem cells to efficiently efflux vital dyes such as Hoechst 33342. During fluoresence activated cell sorting (FACS) such Hoechst-extruding cells appear to the side of Hoechst-dye retaining cells and have thus been termed side population (SP) cells
1.悬浮生长的细胞在培养状态下加入Heochst 33342,终浓度为1μg/ml;帖壁生长的细胞用含有0.02%EDTA的0.25%胰蛋白酶消化成单细胞悬液,离心,弃上清,用1ml全培养液重悬细胞,加入Heochst 33342,终浓度为1μg/ml,37℃孵育7~10min。 2.4℃ 500~1000r/min离心弃去染液。 3.加入1.0ml PI染液,4℃避光染色15min。 4.400目的筛网过滤1次。 5.流式细胞仪分析。
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