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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
有效期1年
- 保存条件:
-20℃至-80℃保存
- 供应商:
翌圣生物科技(上海)股份有限公司
- 库存:
大量
- 英文名:
Recombinant Human Leukemia Inhibitory Factor (Human LIF)
- 规格:
5μg
Product Information
| Product Name |
Cat# |
Size |
Price(¥) |
| Recombinant Human Leukemia Inhibitory Factor (Human LIF) |
92111ES08 |
5μg |
815.00 |
| 92111ES60 |
100μg |
7335.00 |
|
| 92111ES76 |
500μg |
19055.00 |
Product Description
Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. Mature human LIF shares 78%, 82%, 91%, 88 and 87% aa sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively. LIF is multifunctional cytokines with many similar activities. LIF is structurally and functionally related to another cytokine, Oncostatin M (OSM), that binds to the high-affinity LIF receptor but not to the low-affinity LIF receptor. While some of the actions of LIF on neurons resemble those of CNTF, LIF also has broad actions outside of the nervous system that in many cases mimic those of interleukin-6 (IL-6).
Product Properties

| Synonyms |
CDF, DIA, Differentiation-stimulating Factor, D Factor, Emfilermin, HILDA, Melanoma-derived LPL Inhibitor, MLPLI |
| Accession |
|
| GeneID |
|
| Source |
E.coli-derived human LIF, Ser23-Phe202. |
| Molecular Weight |
Approximately 19.7 kDa. |
| AA Sequence |
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF |
| Tag |
None |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity |
>98% by SDS-PAGE and HPLC analyses. |
| Biological Activity |
The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg. Fully biologically active when compared to standard. |
| Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.2 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.
For research use only!
HB220215
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人白血病抑制因子 (LIF)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 LIF 单抗包被于酶标板上,标准品和样品中的 LIF与单抗结合,加入生物素化的抗人 LIF ,形成
amplification reaction. This chapter will focus on the preparation and the use of the 26plex assay with DNA samples for the purpose of human identification.
Analysis of Neurotransmitter Amino Acids by CE-LIF Detection in Biological Fluids
, so that the evaluation of their levels in organisms plays an essential role in physiology. We here report a new capillary electrophoresis (CE) method with laser-induced fluorescence detection by which analytes are resolved and detected in less than 12 min both in human











