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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
德国美天旎生物技术有限公司
- 英文名:
T Cell Activation/Expansion Kit, human
T Cell Activation/Expansion Kit适用于活化或扩增人的T细胞。该试剂盒包含:生物素标记的微磁珠(Anti-Biotin MACSiBead Particles)和生物素化的人CD2, CD3及CD28的抗体。二者结合可以模拟抗原递呈细胞而激活PBMC中或分选出来的T细胞中的静息的T细胞,约培养14天后即可得到T细胞的扩增。
应用
● 活化和扩增PBMC中的静息T细胞。
● 活化和扩增用微磁珠(如CD4 MicroBeads (# 130-045-101)
或CD8 MicroBeads (# 130-045-201))进行阳性分选得到的T细胞;也可以活化用Pan T Cell Isolation Kit II (#
130-091-156),CD4+ T Cell Isolation Kit II (# 130-091-155) 或CD8+ T Cell Isolation Kit II (# 130-091-154)进
行阴性分选得到的T细胞。
● 扩增抗原特异性T细胞。
激活样本
未激活对照
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文献和实验Activation and Expansion of Human Treg Cells
to TCR/CD3 and CD28 for full activation and expansion of human Treg cells. 二、实验试剂 2 ml Dynabeads? Human Treg Expander 2 x 107 beads/ml in phosphate buffered saline (PBS), pH 7.4, w/ 0.1% human serum albumin (HSA). Magnet: Any Dynal MPC Buffer
Human Pregnane X Receptor (hPXR) Activation Assay in Stable Cell Lines
for the application of screening for human PXR activation using commercially available stable cell lines harboring the PXR and a luciferase reporter gene linked to the promoters of the human CYP3A gene. These instructions provide detailed information on how to thaw
Techniques for generating human T-cell clones (TCCs) were first described nearly two decades ago (1 , 2 ). This was a direct consequence of the discovery of T-cell growth factor and the subsequent ability to propagate T-cells over extended











