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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
6X DNA Loading Buffer (Blue) 99962-1
- 库存:
99
- 供应商:
Biotium
- CAS号:
1
6X DNA Loading Buffers contains density agents for loading DNA samples on agarose or polyacrylamide gels. Available with blue or orange tracking dyes.
- Density agents for easy sample loading
- Visible blue or orange dyes for tracking gel electrophoresis
- Compatible with agarose and polyacrylamide gels
- No fluorescent DNA stains
6X DNA Loading Buffer with Blue Tracking Dyes contains two blue tracking dyes that run at ~1.5 kb and ~200 bp in a 1% agarose gel. 6X DNA Loading Buffer (Orange) contains orange tracking dye that runs at ~50 bp in a 1% agarose gel. Unlike blue tracking dyes, orange dye does not shadow DNA bands, and is suitable for use with DNAzure® Blue DNA Gel Stain.
These 6X DNA Loading Buffers do not contain fluorescent DNA dye. See our GelRed® Prestain Plus 6X DNA Loading Dye and 6X GelRed® Prestain Loading Buffers for one-step loading and fluorescent staining. PCR products from EvaGreen® qPCR reactions also can be visualized on gels without additional fluorescent dye.
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文献和实验Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
Electrophoresis DNA Sample Buffer (6X) 0.25% Xylene Cyanol FF 40% (w/v) Sucrose 0.25% Bromophenol Blue
Methylene Blue DNA staining protocol
Methylene Blue DNA staining protocol Protocol: Load 2-5X the amount of DNA that would give bands of moderate intensity on an ethidium bromide stained gel. Typically this is something on the order of 0.5-2.5 µg
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
aqueous DNA into new tube. SOLUTIONS Digestion buffer: 1X PCR Buffer with 0.5% Tween20 / 0.4mg/ml pk stored in aliquots at -20 C. PK: Stored as 20mg/ml aliquots at -20 C; can be refrozen a few times. Methyl Green Staining











