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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
Merck
- 英文名:
6X DNA GEL LOADING BUFFER
- 库存:
1
- 保存条件:
2-8°c
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文献和实验【求助】提质粒后,跑电泳,用10乘Loading Buffer 还是6乘Loading Buffer?
ilovelc999 请问各位,提质粒后,用20微升TE溶解,然后进行电泳,应该用10乘Loading Buffer 还是用6乘Loading Buffer?另外Buffer与质粒溶液一般应以什么比例混合好呢?多谢各位啦! 纯属菜鸟 10乘 5:1的比例 质粒5 肥宝 我是用6X的,1:5,质粒5,不过也没那么严格,一般也就点个小点估计1ul lihuijin017
Radiolabeled sequencing gel preparation, loading, and electrophoresis
buffer, and immediately prior to loading each sample, flush the well with running buffer using gel loading tips. 9. Load 1-2 ul of sample into each well using a Pipetteman with gel-loading tips, and then electrophorese according the following
at room temperature and remelted in a microwave. To prepare samples for electrophoresis, add 1 μl of 6x gel loading dye for every 5 μl of DNA solution. Mix well. Load 5-12 μl of DNA per well (for minigel). Electrophorese at 50-150 volts
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