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- 华纳生物
- WN-13005
- 武汉
- 2025年07月14日
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- 文献和实验
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- 英文名：
pCMV-HA-C
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- 供应商：
武汉华尔纳生物科技有限公司
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Product Introduction
| 品牌 |
华纳生物 | ||||||||||||||||||||||||
| 货号 |
WN-13005 | ||||||||||||||||||||||||
| 规格 |
2ug | ||||||||||||||||||||||||
| 价格 |
询价 | ||||||||||||||||||||||||
| 货期 |
现货 | ||||||||||||||||||||||||
订购信息
质粒图谱 载体描述The pCMV-HA Mammalian Expression Vector expresses proteins containing an N-terminal hemagglutinin (HA) epitope tag. The HA epitope tag is well-characterized and highly immunoreactive. High-level expression in mammalian cells is driven from the human cytomegalovirus immediate early promoter/enhancer (PCMV IE). The vector contains an intron (SV40 splice donor/splice acceptor); the epitope tag; an MCS; and a polyadenylation signal from SV40. This vectors also possesses the ampicillin resistance gene for selection in E. coli. 载体用途To create a fusion of a gene of interest and the HA tag, insert the gene into the MCS in frame with the HA coding sequence. The resulting HA-tagged proteins can be identified with the HA-Tag Polyclonal Antibody (IgG), provided with this vector, or another antibody raised against the HA tag. The epitope tag is also useful for facilitating purification of the protein, identifying associated proteins, characterizing new proteins by immunoprecipitation, and determining subcellular localization. The MCS in this vector is compatible with the MCSs in Clontech's Matchmaker™ Two-Hybrid System Vectors. Compatibility with System 3 Vectors is noted in the MCS diagram. Consult the Vector Information Packet provided with any Matchmaker vector for complete information. After obtaining putative positive clones in your Matchmaker two-hybrid screen, use the pCMV-Myc and pCMV-HA Vectors to verify the interactions identified in yeast directly in mammalian cells. To accomplish this, subclone the selected inserts into the pCMV-Myc Vector and the "bait" insert into the pCMV-HA Vector. Alternatively, clone the "bait" insert into pCMV-Myc and the library inserts into pCMV-HA. To confirm predicted interactions in vivo via coimmunoprecipitation, cotransfect pCMV-Myc with the pCMV-HA Vector into mammalian cells and immunoprecipitate using the c-Myc Monoclonal or HA-Tag Polyclonal Antibody provided with the vectors. |
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武汉华尔纳生物科技有限公司，简称华尔纳生物（Warner Bio），坐 落于武汉生物产业基地·光谷生物城，是一家生物医学资源开发，科研生 产，产品销售集一体的科技型企业。
华尔纳生物（Warner Bio）始终专注于生命科学领域及细胞实验培养 方向，坚持为客户打造优质高效，安全可靠的生命科学产品，我们始终坚 持于以客户为中心、以品质求生存、以价值求发展、以服务求口碑的企业 理念，从而获得全国广大科研用户的一致认可。
团队以高效的服务品质，专业卓越的技术能力构建了华中地区最大的 生物细胞资源典藏中心，经过多年不懈的努力与发展，现与武汉大学、华 中科技大学、武汉协和医院、武汉同济医院、四川大学华西医院、首都医 科大学、中山大学附属第三医院等全国多所高校、医院、研究所单位达成 长期稳定的合作，同时为科研人员提供免费专业的售前售中售后服务，为 您的科研实验保驾护航。
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文献和实验Antisense Tumor Therapy: Activated C-Ha-ras Oncogene in the Mouse
cells are normal cellular genes that have sustained some activating lesion. The ras family of mammalian proto-oncogenes includes three members, termed Ha-ras , Ki-ras , and N-ras , that are found to be activated very often in human solid tumors
birkin 我在尝试转染某种细胞，载体是购买的商业载体（pCMV6)，之前转染HEK293作为预实验，已经用WB证明有效，但是换到现在这种细胞之后就很奇怪，一开始想用短期转，结果WB做不出来，觉得可能是转染率太低，于是又改做稳定转染，用了G418筛选。花了近2个月，细胞稳定下来了，取样做RT-PCR，有相当强的mRNA表达（-RT和对照组转染也做了的，基本没有带，所以不会是污染），谁知做WB还是一点东西都没有……我都快疯了，既然能抗G418，又有大量mRNA
，表达载体pCDNA3，pCDNA3.1(+),pCDNA3.1(-),表达载体pcmv-ha以及表达载体 pCMV-Myc的图谱 http://www.dxy.cn/bbs/actions/archive/post/2019694_0.html pMD18-载体图谱：http://www.dxy.cn/bbs/actions/archive/post/2993026_0.html pGEX-KG载体图谱序列：http://www.dxy.cn/bbs/actions/archive/post
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