- ￥3200
- Proteintech
- CL647-14600
- 2025年10月13日
- IF/ICC, FC (Intra)
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human LC3 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
CoraLite® Plus 647 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
LC3
- 抗体英文名：
CoraLite® Plus 647-conjugated LC3 Polyclonal antibody
- 抗体名：
CoraLite® Plus 647-conjugated LC3 Polyclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | Chloroquine treated HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-14600 targets LC3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag6144 Product name: Recombinant human LC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-125 aa of BC067797 Sequence: MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMGELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | microtubule-associated protein 1 light chain 3 beta |
| 别名 | MAP1LC3B, MAP1A/MAP1B LC3 B, MAP1A/1BLC3, MAP1 light chain 3-like protein 2, LC3B |
| 计算分子量 | 15 kDa |
| GenBank蛋白编号 | BC067797 |
| 基因名称 | LC3B |
| Gene ID (NCBI) | 81631 |
| ENSEMBL Gene ID | ENSG00000140941 |
| RRID | AB_2920226 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9GZQ8 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.
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文献和实验'）2 Fragment（Alexa Fluor® 555 Conjugate）#4413 Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 647 Conjugate）#4414 抗小鼠： Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4408 Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor
647 34079 SuperSignalâ West Pico Chemiluminescent Substrate Trial Kit 50
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