- ￥2800
- Proteintech
- CL555-60011
- 2025年10月13日
- IF/ICC, FC (Intra)
- Mouse
- human, mouse, rat, pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human GATA1 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite®555 Fluorescent Dye
- 适应物种：
human, mouse, rat, pig
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
GATA1
- 抗体英文名：
CoraLite®555-conjugated GATA1 Monoclonal antibody
- 抗体名：
CoraLite®555-conjugated GATA1 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-60011 targets GATA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag1350 Product name: Recombinant human GATA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC009797 Sequence: MEFPGLGSLGTSEPLPQFVDPALVSSTPESGVFFPSGPEGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALPSSLPVPNSAYGGPDFSSTFFSPTGSPLNSAAYSSPKLRGTLPLPPCEARECVNCGATATPLWRRDRTGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCTNCQTTTTTLWRRNASGDPVCNACGLYYKLHHQHYCGGSAQLMRAQSMASRGGVVSFS |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | GATA binding protein 1 (globin transcription factor 1) |
| 别名 | 5E2A8, ERYF1, GATA-1, GATA-binding factor 1, GF1 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC009797 |
| 基因名称 | GATA1 |
| Gene ID (NCBI) | 2623 |
| RRID | AB_2919655 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P15976 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].
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