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- 禾午生物
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- P33534
- 2025年07月12日
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- 文献和实验
- 技术资料
- 保存条件：
-20℃&-80℃
- 保质期：
3个月~12个月
- 英文名：
pEnCMV-PTEN(human)-R173C-3×HA-SV40-Neo
- 库存：
999
- 供应商：
上海禾午生物科技有限公司
- 规格：
干粉&甘油菌
| 别称 | NM_000314.8;10q23del; BZS; CWS1; DEC; GLM2; MHAM;MMAC1; PTEN1; PTENbeta; TEP1 |
| 启动子 | CMV |
| 复制子 | pUC |
| 感受态 | DH5a |
| 培养温度 | 37度 |
| 质粒宿主 | 哺乳细胞 |
| 质粒用途 | 蛋白表达 |
| 片段类型 | ORF |
| 片段物种 | 人 |
| 原核抗性 | Amp |
| 筛选标记 | Neo/G418 |
| pEnCMV-PTEN(human)-R130Q-3×HA-SV40-Neo |
| pEnCMV-PTEN(human)-R233End-3×HA-SV40-Neo |
| pEnCMV-TTLL4(human)-3×FLAG-SV40-Neo(1同义突变) |
| pEnCMV-PTEN(human)-R173C-3×HA-SV40-Neo |
| pEnCMV-PTEN(human)-R173H-3×HA-SV40-Neo |
| pEnCMV-CTNNA2(human)-3(1同义突变)-3×HA-SV40-Neo |
| pEnCMV-Ccnd1(rat)-3×FLAG-SV40-Neo |
| pEnCMV-MRPL23(human)-Linker-EGFP-SV40-Neo |
| PiggyBac-CMV-GRIA1(human)-2(1同义突变)-EF1a-Puro |
| pCMV-HA-UB-K27 |
| pmirGLO-CFL2(mouse)-3UTR-mut |
| pEnCMV-VIM(human)-Linker-GST-SV40-Neo |
| pEnCMV-ZDHHC6(human)-V5-6×His-SV40-Neo |
质粒均可提供详细图谱、序列、测序报告。基因合成 载体构建 病毒包装 稳转株构建
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文献和实验Genetic evidence strongly suggested that a tumor suppressor was located on chromosome 10. During the development of glioblastoma, one copy of chromosome 10 was typically lost (1 ). Cytogenetic and molecular analysis revealed partial
Measurement of PTEN Activity in vivo by Imaging Phosphorylated Akt
This chapter describes an indirect approach to measure PTEN’s lipid phosphatase activity in vivo . PTEN counteracts phosphatidylinositol 3-kinase action in dephosphorylating 3-phosphorylated phosphoinositides. Therefore, PtdIns(3,4,5)P3
18 Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations
Cowden syndrome is a rare dominantly inherited condition with predisposition to benign hamartomatous polyposis of the intestine, as well as malignant tumors of the breast and thyroid, and possibly some other cancer types. Other features include
技术资料
