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- 保存条件：
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
- 规格：
5mg
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文献和实验Blocking of unwanted non-specific staining
is not. Blocking of endogenous peroxidase is done by preincubating the slides in 3 H2O2 1 Sodium Azide PBS 30 minutes several washes This mixture is more effective than 1.5 H2O2 in absolute methanol Ref. Methanol incubation is also a fixation step
, ending with an extensive wash (25ml) in TTBS, containing an extra 0.2 M NaCl. The column is now ready for use. For long term storage, cap bottom of column and wrap Parafilm around the top and store at 4°C (there is azide in the TTBS). Wash Buffer
Replication timing by comparative hybridization
in yeast chromosomes J. Cell Sci. Suppl. 19:51-58. The principle: A non-replicating DNA circle is created in vivo by site-specific pop-out recombination. This non-replicating fragment is then used as an internal control to measure
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