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- 2025年07月14日
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- 保存条件：
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
- 规格：
5mg
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文献和实验Blocking of unwanted non-specific staining
is not. Blocking of endogenous peroxidase is done by preincubating the slides in 3 H2O2 1 Sodium Azide PBS 30 minutes several washes This mixture is more effective than 1.5 H2O2 in absolute methanol Ref. Methanol incubation is also a fixation step
, ending with an extensive wash (25ml) in TTBS, containing an extra 0.2 M NaCl. The column is now ready for use. For long term storage, cap bottom of column and wrap Parafilm around the top and store at 4°C (there is azide in the TTBS). Wash Buffer
protocol for conjugation of proteins to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used
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