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上海臻诺生物科技有限公司
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文献和实验, 1.4 numerical aperture oil objective and a CCD camera with a 1,317 × 1,035 Kodak chip (pixel size 6.8 μm × 6.8 μm; Princeton Instruments Inc.). Blue fluorescence is detected using the excitation filter 350/50 D, the beamsplitter 400 DCLP and the emission filter
flour at -40oC until extraction. Add 20 ml preheated (to about 70oC by micro-oven) extraction buffer A and 50 ul proteinase K (20 mg/ml), and then stir gently using a spatula. Incubate at 55oC for 30 min. Add 20 ml chloroform : isoamylalcohol (24
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
(Vector Laboratories PK 6100) (optional, see Step 30) Wash solution 1 Wash solution 2 Equipment Aluminum foil Diamond pen Eye protection (see Step 21) Filter paper (e.g., Whatman 3MM) Humid chamber for slide
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