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文献和实验CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA
volume of 3M Na Acetate, mixing with pipet. Add 2.5 X (original) vol 100% EtOH to ppt DNA, vortex gently. Spin 30 mins at 14K rpm, 4 C. Decant supernatant; blot dry, being careful to avoid DNA pellet. Add 10 ul of MM1/H20 mix (70% MM1/30
CGH of DIRECT LABELED TEST DNA vs NORMAL DNA
. Let rubber cement dry 5 mins on warmer. Incubate for 2-3 days at 37 C in a humid chamber. DAY 2: WASHES AND STAINING Remove rubber cement. Slide coverslips off gently. Wash 3X for 12 mins each at 45 C in 50
Planar Lipid Bilayer Experiment平面脂双层实验
on the top and connect it to the voltmeter ( while the two electrodes are in a cell with some salt solution), check to see voltage applied = what you see on voltmeter. Change battery (2 x 9V) if it?s off.2、Don?t touch calibrated knob on power box.3、Set rise time to 10
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