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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse F-box only protein 32 (Fbxo32)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse F-box only protein 32 (Fbxo32)品名简称:
Recombinant Mouse Fbxo32 protein货号:
CSB-EP887410MO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Fbxo32Uniprot No.:
Q9CPU7别名:
Atrogin-1 (Muscle atrophy F-box protein) (MAFbx)种属:
Mus musculus (Mouse)蛋白长度:
Full Length来源:
E.coli分子量:
48.9 kDa表达区域:
1-355aa氨基酸序列:
MPFLGQDWRSPGQSWVKTADGWKRFLDEKSGSFVSDLSSYCNKEVYSKENLFSSLNYDVAAKKRKKDIQNSKTKTQYFHQEKWIYVHKGSTKERHGYCTLGEAFNRLDFSTAILDSRRFNYVVRLLELIAKSQLTSLSGIAQKNFMNILEKVVLKVLEDQQNIRLIRELLQTLYTSLCTLVQRVGKSVLVGNINMWVYRMETILHWQQQLNSIQISRPAFKGLTITDLPVCLQLNIMQRLSDGRDLVSLGQAAPDLHVLSEDRLLWKRLCQYHFSERQIRKRLILSDKGQLDWKKMYFKLVRCYPRREQYGVTLQLCKHCHILSWKGTDHPCTANNPESCSVSLSPQDFINLFKF蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Substrate recognition component of a SCF E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.功能2:
/文献:
"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005)研究领域:
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文献和实验机制及潜在干预策略。研究团队发现RNF115在心力衰竭患者及压力超负荷小鼠心脏中表达显著上调,其受转录因子c-JUN调控,通过与细胞骨架蛋白SPTBN1相互作用并促进其K48型泛素化降解,导致F-actin解聚、G-actin增多,进而使YAP去磷酸化入核,激活肥厚相关基因表达。心肌细胞特异性敲除RNF115可显著减轻心肌肥厚和心功能障碍。此外,团队还合成了RNF115小分子抑制剂DTD,证实其低毒且能有效改善小鼠心肌肥厚和心功能障碍,为心力衰竭的临床治疗提供了新型干预靶点和理论
Determination of Muscle Fiber Type in Rodents
and have different metabolic and enzymatic activities, which can be used as surrogate markers to identify the different fiber types. Pathological changes affecting the muscle, such as denervation, muscle disuse, and atrophy not only manifest on a functional level
and trochlear motoneurons leads to altered extraocular muscle development in the Wnt‐1 null mutant mouse. Brain Res. Dev. Brain Res. 100:121‐126. Priest, J.R., Girirajan, S., Vu, T.H
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