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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Soluble calcium-activated nucleotidase 1 (CANT1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥10872.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2514.0 |
| 规格: | 20μg | 产品价格: | ¥1344.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
CANT1Uniprot No.:
Q8WVQ1别名:
Apyrase homologPutative MAPK-activating protein PM09Putative NF-kappa-B-activating protein 107种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
39.4 kDa表达区域:
80-397aa氨基酸序列:
GQAPANWYNDTYPLSPPQRTPAGIRYRIAVIADLDTESRAQEENTWFSYLKKGYLTLSDSGDKVAVEWDKDHGVLESHLAEKGRGMELSDLIVFNGKLYSVDDRTGVVYQIEGSKAVPWVILSDGDGTVEKGFKAEWLAVKDERLYVGGLGKEWTTTTGDVVNENPEWVKVVGYKGSVDHENWVSNYNALRAAAGIQPPGYLIHESACWSDTLQRWFFLPRRASQERYSEKDDERKGANLLLSASPDFGDIAVSHVGAVVPTHGFSSFKFIPNTDDQIIVALKSEEDSGRVASYIMAFTLDGRFLLPETKIGSVKYEG蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP. Involved in proteoglycan synthesis.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
Epigenetics and Nuclear SignalingReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
Cloning, expression, and characterization of a soluble calcium-activated nucleotidase, a human enzyme belonging to a new family of Extracellular domain nucleotidases.Smith T., Hicks-Berger C., Kim S., Kirley T.Arch. Biochem. Biophys. 406:105-115(2002)Function:
Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Characterization of P1 (Adenosine) Purinoceptors
a Saturation binding parameters (K d and B max ) for A 1 and A 2A are for rat brain. The A 2B and A 3 binding parameters (K d , B max , and K i ) are for the recombinant human receptors.
,真正赋予生物体的表现型的却是基因的产物。由于存在众多的蛋白质结构、功能和降解的翻译后调节机制,仅靠对于基因的认识甚至已难以圆满解释生物体系的复杂性。从筛选的角度来讲,由组织分泌或释放入血流中也主要是蛋白[6,7]。然而尽管经过了数十年的倾心研究,已鉴定的癌症生物标记物中,证明确实具有临床意义的也不过寥寥数种蛋白、且全是血浆蛋白(如前列腺特异性抗原[PSA]、癌胚抗原[CEA]、癌抗原125 [CA125]和甲状腺球蛋白),常常需要结合其他诊断工具才能预测患者对治疗的反应性、复发和生存率、对病情进行
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