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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human metapneumovirus Phosphoprotein (P)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human metapneumovirus Phosphoprotein (P)品名简称:
Recombinant Human metapneumovirus Phosphoprotein货号:
CSB-EP804307HDAM规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
PUniprot No.:
Q8B9Q8别名:
Protein P种属:
Human metapneumovirus (strain CAN97-83) (HMPV)蛋白长度:
Full Length来源:
E.coli分子量:
40.2 kDa表达区域:
1-294aa氨基酸序列:
MSFPEGKDILFMGNEAAKLAEAFQKSLRKPSHKRSQSIIGEKVNTVSETLELPTISRPTKPTILSEPKLAWTDKGGAIKTEAKQTIKVMDPIEEEEFTEKRVLPSSDGKTPAEKKLKPSTNTKKKVSFTPNEPGKYTKLEKDALDLLSDNEEEDAESSILTFEERDTSSLSIEARLESIEEKLSMILGLLRTLNIATAGPTAARDGIRDAMIGIREELIADIIKEAKGKAAEMMEEEMNQRTKIGNGSVKLTEKAKELNKIVEDESTSGESEEEEELKDTQENNQEDDIYQLIM蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Acts as a cofactor that serves both to stabilize the protein L and to place the polymerase complex on the N:RNA template.功能2:
/文献:
"Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains." Bastien N., Normand S., Taylor T., Ward D., Peret T.C., Boivin G., Anderson L.J., Li Y. Virus Res. 93:51-62(2003)研究领域:
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文献和实验三句话读懂一篇 CNS:咖啡竟可降低心律失常风险;转入肥胖基
300 万人住院,目前仍无特异性治疗药物和预防疫苗。2021 年 7 月 20 日,复旦大学上海公共卫生临床中心鄢慧民和姜世勃教授团队在 Cell Reports 杂志上发表研究论文 A safe and effective mucosal RSV vaccine in mice consisting of RSV phosphoprotein and flagellin variant。该团队研发出新型 RSV 黏膜疫苗 P-KFD1,抗原为一种重组融合蛋白。P-KFD1 鼻内免疫小鼠,能够显著
-femtomolar level if enough radioactivity can be incorporated into the phosphoprotein of interest. In our hands, this can be as little as 1,000 cpm (not ideal). Briefly, a 32 P-labeled protein is digested with a protease and the resulting phosphopeptides
质芯片检测。此外,肽芯片或基于质谱的方法[ 20,21] 也可以用于这方面。 我们在此介绍基于蛋白质芯片技术的蛋白磷酸化筛选方法和高通量确定蛋白激酶底物的方法。我们已成功地用这种筛选工具鉴定大麦酪蛋白激酶 2α ( CK 2α) 和不同的拟南芥丝裂原活化蛋白(MAP) 激酶 [23] 的新靶标。我们这个方法使用本书第 28 章详细描述的植物蛋白质芯片(见第 28 章)。在放射性【 γ33 磷】三磷酸腺苷存在的条件下,用可溶和具有活性的激酶孵育芯片。通过磷屏成像仪或 X 射线胶片检测到的放射性信号,对可能
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