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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Neuropilin-1 (NRP1) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Neuropilin-1 (NRP1) (Active)品名简称:
Recombinant Human NRP1 protein (Active)货号:
CSB-MP016091HU规格:
20μg价格:
696LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 95% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Measured by its binding ability in a functional ELISA. Immobilized human VEGF165 (CSB-MP025833HU(F4)) at 2 μg/ml can bind human NRP1, the EC50 is 22.68-34.55 ng/ml.基因名:
NRP1Uniprot No.:
O14786别名:
/种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein of Isoform 2来源:
Mammalian cell分子量:
98.8 kDa表达区域:
22-644aa氨基酸序列:
FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFVPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK蛋白标签:
C-terminal hFc1-tagged产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
/研究领域:
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文献和实验10.14 Nature:在人类癌症基因组非编码区域中鉴别出关键的致癌突变
来激活 FAK/p130Cas 通路,从而促进结直肠癌细胞的迁移、侵袭和转移。此外,形成 NRP1 突变体/MET/β1-整联蛋白复合体的结肠癌细胞还会对抑制 MET 酪氨酸激酶活性的药物出现耐受,这就表明,携带这些突变体的结直肠癌患者很有可能并不会对 MET 靶向性疗法产生反应。原文检索:N-glycosylation-defective splice variants of neuropilin-1 promote metastasis by activating endosomal
也可以从其他物种中去除白蛋白。适用于处理 25 或 50 个样本。Figure 1: 2D analysis of whole human serum before (left) and after(right) treatment with AlbminOUT™. 产品特点: ● 从样本中不到 10 分钟的时间内去除白蛋白 ● 基于白蛋白和 Cibachron 蓝色染料的结合 ● 每个柱子的结合力大于 2 mg 的人白蛋白 ● 从 5-50
X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏蛋白
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