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文献和实验Alternative Cell Culture Systems
1.Cell Culture Scale-up Systems Most tissue culture is performed on a small scale where relatively small numbers of cells are required for experiments. At this scale cells are usually grown in T flasks ranging from 25cm2 to 175cm2. Typical cell
gasketted centrifuge bottles. Spin at 2600rpm in a GSA rotor for 20 minutes. Aspirate the fly bodies and oily layer off the top of the bottle. Transfer liquid to new 250ml gasketted centrifuge bottles. Spin again at 3000rpm in a GSA rotor for 30
The Do and Don’t of Cell Culture
Given below are a few of the essential "do’s and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipment (PPE). Many of them are common sense and apply to all laboratory areas
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