纯度:>98% as determined by SDS-PAGE. Ni-NTA chromatography.
生物活性:Measure by its ability to induce proliferation in NIH-3T3 mouse embryonic fibroblast cells in the presence of mouse Klotho beta and heparin.The ED50 for this effect is < 2 μg/mL.
内毒素检测:<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列:AYPIPDSSPLLQFGGQVRQRYLYTDDD QDTEAHLEIREDGTVVGAAHRSPESLL ELKALKPGVIQILGVKASRFLCQQPDG ALYGSPHFDPEACSFRELLLEDGYNVY QSEAHGLPLRLPQKDSPNQDATSWG PVRFLPMPGLLHEPQDQAGFLPPEPP DVGSSDPLSMVEPLQGRSPSYAS with polyhistidine tag at the N-terminus