- 询价
- Biotium
- 美国
- 21005-1L
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
6个月
- 英文名：
One-Step Lumitein UV Protein Gel Stain, 1X 21005-1L
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
One-Step Lumitein™ UV Protein Gel Stain is a ready-to-use luminescent red protein gel stain optimized for use on UV transilluminators. With no fixation or wash steps required, One-Step Lumitein™ UV is a great choice for convenience and safety. One-Step Lumitein™ UV can detect as little as approximately 1-10 ng of protein per band.
- One-step staining procedure, no fixation required
- Non-toxic formulation is safe for easy disposal
- Sensitive protein detection down to 1-10 ng
- Optimized for use with a UV transilluminator
- 1 L and 4 L sizes
- Store at 4°C
Fast and simple procedure
One-Step Lumitein™ UV gel staining requires only a single 5-30 minute staining step without fixation. Destaining is optional. One-Step Lumitein™ UV can detect as little as approximately 1-10 ng of protein per band depending on the staining method used, although staining intensity varies between proteins. Staining is fully compatible with mass spectrometry and Edman-based sequencing. Gels stained with One-Step Lumitein™ UV Protein Gel Stain can be visualized with a variety of different UV-based fluorescence imaging systems.
The results obtained with One-Step Lumitein™ UV are comparable to those with Oriole™ Fluorescent Gel Stain; however, One-Step Lumitein™ UV has a more convenient protocol, does not contain hazardous solvents, and does not cause gel shrinkage. Furthermore, One-Step Lumitein™ costs significantly less than other fluorescent protein gel stains. One-Step Lumitein™ UV Protein Gel Stain is available in 1 liter bottle or 4 liter Cubitainer® sizes.
One-Step Lumitein™ UV is non-toxic
One-Step Lumitein™ UV Protein Gel Stain offers safer handling and ease of disposal, because it is an aqueous-based solution that does not contain hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.
Please visit our Protein Gel Stains technology page to learn about our other safe and sensitive protein gel stains such as One-Step Lumitein™ and One-Step Blue™, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue staining.
Still deciding? Request a free sample of any of our One-Step protein gel stains.
Request a Free Sample
| Product | Replacement for | Catalog number | Unit size | Product protocol | Safety report |
|---|---|---|---|---|---|
| One-Step Blue® | Coomassie stain | 21003-1L | 1L | PI-21003 | One-Step Gel Stains Safety Report |
| One-Step Lumitein™ | SYPRO® Ruby Protein Gel Stain | 21004-1L | 1L | PI-21004 | |
| 21004-4L | 4L | ||||
| One-Step Lumitein™ UV | Oriole™ and Flamingo™ Protein Gel Stains | 21005-1L | 1L | PI-21005 | |
| 21005-4L | 4L |
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文献和实验水在小烧杯内混合，加入过硫酸铵和TEMED，轻轻搅拌使其混匀。 （2）将浓缩胶溶液用吸管加至分离胶上面，直至前玻璃板的上缘。 （3）迅速将点样梳插入凝胶内，直至梳齿的底部与前玻璃板的上缘平齐。待凝胶聚合 (约30min) 后，小心拔出点样梳。 5. 预电泳 将1X电泳缓冲液加入内外电泳槽，使凝胶的上下端均能浸泡在电泳缓冲液内；接通电源，上槽为负极，下槽为正极，80V预电泳3～5min。 6. 样品制备/上样/电泳 将20?滋l蛋白质样品与20?滋l 2×上样缓冲液，在Eppendorf
目的要求 学会SDS-聚丙烯酰胺凝胶电泳法原理。 掌握用SDS-聚丙烯酰胺凝胶电泳法测定蛋白质分子量的操作技术。 实验原理 SDS是十二烷基硫酸钠（sodium dodecyl sulfate）的简称，它是一种阴离子表面活性剂，加入到电泳系统中能使蛋白质的氢键
，将胶模放进电泳槽。 (9)向电泳槽倒入1X 电泳缓冲液，没过凝胶5mm。 (10)小心地拔掉梳子。 (11)用20uL的枪吸取DNA溶液，缓缓注入点样孔。 (12)按marker、限制酶酶切的DNA、提取的DNA依次点样 (13)接通电源，电泳。 (14)电泳完毕，取出胶模，将胶置入EB染色液中染色0.5小时。 (15)用搓板取出胶，置于紫外灯下观察。 本文总结了DNA的限制性酶切和DNA的琼脂
