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One-Step 紫外蛋白质凝胶染色法, 1X 21005-

4L
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  • 询价
  • Biotium已认证
  • 美国
  • 21005-4L
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      4°C

    • 保质期

      6个月

    • 英文名

      One-Step Lumitein UV Protein Gel Stain, 1X 21005-4L

    • 库存

      99

    • 供应商

      Biotium

    • CAS号

      1

    One-Step Lumitein™ UV Protein Gel Stain is a ready-to-use luminescent red protein gel stain optimized for use on UV transilluminators. With no fixation or wash steps required, One-Step Lumitein™ UV is a great choice for convenience and safety. One-Step Lumitein™ UV can detect as little as approximately 1-10 ng of protein per band.

    • One-step staining procedure, no fixation required
    • Non-toxic formulation is safe for easy disposal
    • Sensitive protein detection down to 1-10 ng
    • Optimized for use with a UV transilluminator
    • 1 L and 4 L sizes
    • Store at 4°C

    Fast and simple procedure

    One-Step Lumitein™ UV gel staining requires only a single 5-30 minute staining step without fixation. Destaining is optional. One-Step Lumitein™ UV can detect as little as approximately 1-10 ng of protein per band depending on the staining method used, although staining intensity varies between proteins. Staining is fully compatible with mass spectrometry and Edman-based sequencing. Gels stained with One-Step Lumitein™ UV Protein Gel Stain can be visualized with a variety of different UV-based fluorescence imaging systems.

    The results obtained with One-Step Lumitein™ UV are comparable to those with Oriole™ Fluorescent Gel Stain; however, One-Step Lumitein™ UV has a more convenient protocol, does not contain hazardous solvents, and does not cause gel shrinkage. Furthermore, One-Step Lumitein™ costs significantly less than other fluorescent protein gel stains. One-Step Lumitein™ UV Protein Gel Stain is available in 1 liter bottle or 4 liter Cubitainer® sizes.

    One-Step Lumitein™ UV is non-toxic

    One-Step Lumitein™ UV Protein Gel Stain offers safer handling and ease of disposal, because it is an aqueous-based solution that does not contain hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

    产品细节图片1
    Red fluorescent One-Step Lumitein™ UV Protein Gel Stain is designed for imaging using a UV transilluminator. It is an alternative to Oriole™ Fluorescent Gel Stain, but does not contain hazardous solvents and does not cause gel shrinkage.

    Please visit our Protein Gel Stains technology page to learn about our other safe and sensitive protein gel stains such as One-Step Lumitein™ and One-Step Blue™, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue staining.

    Still deciding? Request a free sample of any of our One-Step protein gel stains.

     

    Request a Free Sample

     

    Product Replacement for Catalog number Unit size Product protocol Safety report
    One-Step Blue® Coomassie stain 21003-1L 1L PI-21003 One-Step Gel Stains Safety Report
    One-Step Lumitein™ SYPRO® Ruby Protein Gel Stain 21004-1L 1L PI-21004
    21004-4L 4L
    One-Step Lumitein™ UV Oriole™ and Flamingo™ Protein Gel Stains 21005-1L 1L PI-21005
    21005-4L 4L
    One-Step Blue is a registered trademark of Biotium. Lumitein and its related technologies are covered by US and international patents. SYPRO is a registered trademark of Molecular Probes, Inc. Oriole and Flamingo are trademarks of Bio-Rad Laboratories. Cubitainer is a registered trademark of Hedwin Corporation.

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    图标文献和实验
    相关实验
    • 蛋白质SDS-聚丙烯酰胺凝胶电泳

      水在小烧杯内混合,加入过硫酸铵和TEMED,轻轻搅拌使其混匀。 (2)将浓缩胶溶液用吸管加至分离胶上面,直至前玻璃板的上缘。 (3)迅速将点样梳插入凝胶内,直至梳齿的底部与前玻璃板的上缘平齐。待凝胶聚合 (约30min) 后,小心拔出点样梳。 5. 预电泳 将1X电泳缓冲液加入内外电泳槽,使凝胶的上下端均能浸泡在电泳缓冲液内;接通电源,上槽为负极,下槽为正极,80V预电泳3~5min。 6. 样品制备/上样/电泳 将20?滋l蛋白质样品与20?滋l 2×上样缓冲液,在Eppendorf

    • DNA的限制性酶切与琼脂糖电泳

      ,将胶模放进电泳槽。 (9)向电泳槽倒入1X 电泳缓冲液,没过凝胶5mm。 (10)小心地拔掉梳子。 (11)用20uL的枪吸取DNA溶液,缓缓注入点样孔。 (12)按marker、限制酶酶切的DNA、提取的DNA依次点样 (13)接通电源,电泳。 (14)电泳完毕,取出胶模,将胶置入EB染色液中染色0.5小时。 (15)用搓板取出胶,置于紫外灯下观察。 本文总结了DNA的限制性酶切和DNA的琼脂

    • 免疫印迹实验相关原理介绍

      免疫印迹(Western blot)简介和原理 免疫印迹用于鉴定能够与特异性抗体相互作用的大分子抗原(一般为蛋白质)并测定抗原的大小。蛋白质首先通过 SDS 聚丙烯酰胺凝胶电泳分离,再通过电泳转移到固相支持物上,固相支持物包括硝酸纤维素膜,聚偏乙烯二氟(PVDF)膜和阳离子尼龙膜等。首先把膜上未反应的位点封闭起来以抑制抗体的非特异性吸附,这样固定的蛋白即可与特异性的多克隆或单克隆抗体相互作用。最后通过放射,生色或化学发光的方法进行定位。 实验常规试剂 1.0 mol/L Tris•HCl

    图标技术资料

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    资料下载:

    PI-21005.pdf 附 (下载 0 次)

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