- 询价
- Cell Signaling Technology
- 3701
- USA
- 2025年12月20日
- W
- Rabbit
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-GRB10 (Tyr67) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Tyr67 of human GRB10
- 应用范围：
W
- 宿主：
Rabbit
- 保质期：
详见说明书
- 供应商：
CST
- 库存：
大量
- 适应物种：
H
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Transfected Only | 76 (Transfected only) | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-GRB10 (Tyr67) Antibody detects transfected GRB10 only when phosphorylated at tyrosine 67. The antibody does not cross-react with phosphorylated GRB7 or GRB14. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr67 of human GRB10. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from COS cells, untransfected (lane 1) or transfected with either wild-type GRB10 (lanes 2-4) or GRB10 Y67G mutant (lanes 5-7), untreated or treated with EGF or insulin as indicated, using Phospho-GRB10 (Tyr67) Antibody (upper) or GRB10 Antibody #3702 (lower). (Plasmids provided by Dr. F. Liu, Dept. of Pharmacology and Biochemistry, University of Texas Health Science Center.) |
| Background | The GRB7 family of adaptor proteins consist of GRB7, GRB10 and GRB14, which all contain an amino-terminal proline-rich SH3 binding domain, followed by PH, PBS and SH2 domains. Each member of the family has several splice variants (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules such as Raf, Akt and Nedd4 (1,2). Although it was originally thought that GRB10 is exclusively phosphorylated at serine residues (3), Src kinase family members have been shown to phosphorylate GRB10 at Tyr67 (4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验baibai17 今天开题，导师问了个问题：ki67为什么叫ki67？当场傻住······恳请各位老师指教！谢谢。 keavin521 The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67 (Gerdes et al., 1983),which was generated by immunizing mice
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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