- 询价
- Cell Signaling Technology
- 2921
- USA
- 2025年11月14日
- W
- Rabbit
- H,R,M
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-Cyclin D1 (Thr286) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Thr286 of human cyclin D1
- 应用范围：
W
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
H,R,M
- 保质期：
详见说明书
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H R (M) | Endogenous | 36 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Cyclin D1 (Thr286) Antibody detects endogenous levels of cyclin D1 only when phosporylated at threonine 286. The antibody does not cross-react with other cyclin D family members at physiological levels. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr286 of human cyclin D1. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from SK-N-MC and C6 cells, untreated or EGF-treated, using Phospho-Cyclin D1 (Thr286) Antibody (upper) or Cyclin D1 mouse mAb #2926 (lower). Western Blotting
Western blot analysis of extracts from SK-N-MC cells, untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-Cyclin D1 (Thr286) Antibody (upper) or Cyclin D1 Mouse mAb #2926 (lower). |
| Background | Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4). Ubiquitination of cyclin D1 is enhanced by phosphorylation at Thr286 by glycogen synthase kinase 3beta (GSK-3beta) (4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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