- 询价
- Cell Signaling Technology
- 3693
- USA
- 2025年08月08日
- W, IF-IC, F
- Rabbit
- M
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Perforin Antibody (Mouse Specific)
- 抗原：
synthetic peptide corresponding to residues surrounding leucine 349 of mouse perforin
- 应用范围：
W, IF-IC, F
- 宿主：
Rabbit
- 供应商：
CST
- 库存：
大量
- 适应物种：
M
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC F | M | Endogenous | 70-75 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Perforin Antibody (Mouse Specific) detects endogenous levels of total Perforin protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding leucine 349 of mouse perforin. Western Blotting
Western blot analysis of extracts from CTLL-2 cells, using Perforin Antibody (Mouse Specific). Flow Cytometry
Flow cytometric analysis of untreated CTLL-2 cells, using Perforin Antibody (Mouse Specific) (blue) compared to a nonspecific negative control antibody (red). IF-IC
Immunofluorescent analysis of CTLL-2 cells showing granule staining, using Perforin Antibody (Mouse Specific). |
| Background | Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells (4). Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
Screening of Antigen-Specific Antibody-Secreting Cells
Screening of antigen-specific antibody-producing cells is a key step for obtaining antigen-specific monoclonal antibodies. In murine system, hybridoma between B-lymphocytes and myeloma cells is used to screen and produce antigen-specific
Antibody Targeting of Nanoparticles to Tumor-Specific Receptors: Immunoliposomes
Immunoliposomes generated by coupling of antibodies to the liposomal surface allow for an active tissue targeting, e.g., through binding to tumor cell-specific receptors. Instead of whole antibodies, single-chain Fv fragments (scFv
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