- $589
- AssayComplete™
- 详见说明
- 93-0563R20B
- 2025年06月14日
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文献和实验Human Embryonic Stem (ES) Cell Protocols――Matrigel Aliquoting and Plating
Aliquoting Matrigel: Day one: Put the sterilized tip box (either 200 ml or 1000 ml tips), sterilized eppendorf tube container, and appropriate pipettor in -20 °C freezer. Thaw the Matrigel bottle on ice in the 4 °C fridge
Extraction of DNA using DNAzol Reagent
from solid and liquid samples of animal, plant, yeast, and bacterial origin. The DNAzol® Reagent procedure is based on the use of a novel guanidine-detergent lysing solution which permits selective precipitation of DNA from a cell lysate. Since first proposed
Extraction of DNA using DNAzol® Reagent
the culture plate and gently pipet the lysate into an assay tube. Cell Pellets or Suspensions: Add 1 ml of DNAzol® Reagent to 1-3 × 107 cells, either in pellet or in suspension (volume 100 µl), pellet the cells and wash them with 0.9% NaCl. Pellet
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