- 询价
- Biotium
- 美国
- 30029-T
- 2025年07月13日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 保存条件：
4°C
- 保质期：
6个月
- 英文名：
NUCVIEW 488 CASPASE 3 ASSAY KIT, TRIAL SIZE 30029-T
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
NucView® 488 Caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time. See a video of NucView® 488 in action.
Features
- Rapid, no-wash, endpoint or real-time assays
- Non-toxic, allowing multi-day experiments to be performed
- For flow cytometry, microscopy or live cell imaging systems
- Dual detection of caspase activity and nuclear morphology
- Formaldehyde fixable
Kit Components
- NucView® 488 Caspase-3 substrate, 0.2 mM in DMSO
- Caspase-3 inhibitor Ac-DEVD-CHO, 2 mM in DMSO
Spectral Properties
- Ex/Em 500/530 nm (after cleavage, with DNA)
Unlike conventional caspase assays, NucView® 488 Caspase-3 substrate detects caspase-3 activity within individual intact cells without cell lysis or inhibition of caspase-3 activity. The substrate consists of a fluorogenic DNA dye and a DEVD substrate moiety specific for caspase-3. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the NucView® 488 caspase-3 substrate is bi-functional, allowing detection of intracellular caspase-3 activity and visualization of changes in nuclear morphology during apoptosis. The fluorescent staining produced in response to caspase-3 activity is compatible with subsequent fixation and permeabilization for immunostaining.
The kit contains NucView® 488 Caspase-3 substrate and a caspase-3 inhibitor Ac-DEVD-CHO. The number of assays per kit is based on 5 uM substrate concentration in 200 uL assay volume; actual number of assays may vary based on concentration and staining volume used.
We also offer NucView® 488 Caspase-3 Substrate at 1 mM in DMSO (10402) or 1 mM in PBS (10403), for customers who wish to control the concentration of DMSO in their assay. Note that in cell types that are not sensitive to DMSO, the presence of DMSO during substrate incubation can enhance signal. See our full selection of NucView® substrates and kits.
NucView® enzyme substrate technology is covered by U.S. Patents.风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
