- 询价
- Biotium
- 美国
- 30029-T
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
6个月
- 英文名：
NUCVIEW 488 CASPASE 3 ASSAY KIT, TRIAL SIZE 30029-T
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
NucView® 488 Caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time. See a video of NucView® 488 in action.
Features
- Rapid, no-wash, endpoint or real-time assays
- Non-toxic, allowing multi-day experiments to be performed
- For flow cytometry, microscopy or live cell imaging systems
- Dual detection of caspase activity and nuclear morphology
- Formaldehyde fixable
Kit Components
- NucView® 488 Caspase-3 substrate, 0.2 mM in DMSO
- Caspase-3 inhibitor Ac-DEVD-CHO, 2 mM in DMSO
Spectral Properties
- Ex/Em 500/530 nm (after cleavage, with DNA)
Unlike conventional caspase assays, NucView® 488 Caspase-3 substrate detects caspase-3 activity within individual intact cells without cell lysis or inhibition of caspase-3 activity. The substrate consists of a fluorogenic DNA dye and a DEVD substrate moiety specific for caspase-3. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the NucView® 488 caspase-3 substrate is bi-functional, allowing detection of intracellular caspase-3 activity and visualization of changes in nuclear morphology during apoptosis. The fluorescent staining produced in response to caspase-3 activity is compatible with subsequent fixation and permeabilization for immunostaining.
The kit contains NucView® 488 Caspase-3 substrate and a caspase-3 inhibitor Ac-DEVD-CHO. The number of assays per kit is based on 5 uM substrate concentration in 200 uL assay volume; actual number of assays may vary based on concentration and staining volume used.
We also offer NucView® 488 Caspase-3 Substrate at 1 mM in DMSO (10402) or 1 mM in PBS (10403), for customers who wish to control the concentration of DMSO in their assay. Note that in cell types that are not sensitive to DMSO, the presence of DMSO during substrate incubation can enhance signal. See our full selection of NucView® substrates and kits.
NucView® enzyme substrate technology is covered by U.S. Patents.风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验设计的底物使该试剂盒可用于检测活细胞内总的Caspase 的活性。 3、Caspase Flow Cytometric Apoptosis Assay Kit 目录号：QIA78 包装：100T/Kit 本试剂盒为您提供了一种快速而灵敏的活细胞内的Caspase 活性的流式细胞检测方法，此方法的原理主要是Caspase 家族的酶可以切割报告底物D2R（目录号：251300）而产生可被激发出荧光的物质罗丹明（Rhodamine），其荧光强度可以用流式细胞仪进行定量检测（激发
were labeled with YO-PRO-1 dye (green) (Y3603). Necrotic cells were detected with propidium iodide (red) 10 µM喜树碱诱导的Jurkat cells（慢淋。G1检测点缺陷细胞） 凋亡。细胞然后经 Vybrant Apoptosis Assay Kit #4 (V13243).处理。凋亡细胞核被YO-PRO-1绿染，坏死细胞被pi红染。 Jurkat human T-cell leukemia
plasmid (Addgene, Cambridge, MA, USA). 11. pUC19 plasmid (NEB). 2.3 Genome Modifi cation and Homologous Recombination Assay Reagents 1. 1× PBS. 2. QuickExtract? DNA extraction kit (Epicentre). 3. SURVEYOR Mutation Detection Kit
