- 询价
- Cell Signaling Technology
- 2022
- USA
- 2025年08月28日
- W
- Rabbit
- H
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
IL-1β Antibody
- 抗原：
synthetic peptide corresponding to the amino-terminal sequence of the 17 kDa mature form of human IL-1 beta
- 应用范围：
W
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 库存：
大量
- 适应物种：
H
- 保质期：
详见说明书
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 17, 31 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | IL-1 beta Antibody detects endogenous levels of both the 31 kDa precursor and 17 kDa mature form of IL-1 beta. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of the 17 kDa mature form of human IL-1 beta. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from THP-1 cells, untreated or lipopolysaccharide (LPS)-treated (10 ug/ml for 24 hours), using IL-1beta Antibody. Cells were differentiated with 0.5 uM TPA for 3 hours. Cells were washed and plated onto 24-well plates at a density of 4 x 10e5 cells per well and left to adhere overnight prior to LPS treatment with the addition of 5 mM ATP for 1 hour to stimulate release of IL-1 beta . |
| Background | Interleukin-1beta (IL-1beta), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1beta is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1beta is a good indicator of caspase-1 activity.
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验正文炎症等免疫研究中 IL-1 家族的成员是重要的研究对象。这些促炎症和抗炎症因子在有机体应对病原感染和损伤时扮演了重要作用。IL-1β 有其自身鲜明的特点，实验前需要多了解。下面让我们来一一解读。1. 靶标生物学特点IL-1β 是 IL-1 家族众多成员中研究的最为深入的成员。包括免疫细胞（如单核细胞和巨噬细胞）在内的很多细胞都可以产生并分泌。IL-1β 的释放大致分为 3 个步骤：i）无活性前体的产生；ii）前体的成熟活化；iii）活化蛋白向胞外的分泌。众多刺激都会诱导这个过程，可分为病原
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
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