IL-1β Antibody

2022:

Western Blotting

IL-1 beta Antibody detects endogenous levels of both the 31 kDa precursor and 17 kDa mature form of IL-1 beta.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of the 17 kDa mature form of human IL-1 beta. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from THP-1 cells, untreated or lipopolysaccharide (LPS)-treated (10 ug/ml for 24 hours), using IL-1beta Antibody. Cells were differentiated with 0.5 uM TPA for 3 hours. Cells were washed and plated onto 24-well plates at a density of 4 x 10e5 cells per well and left to adhere overnight prior to LPS treatment with the addition of 5 mM ATP for 1 hour to stimulate release of IL-1 beta .

Interleukin-1beta (IL-1beta), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1beta is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1beta is a good indicator of caspase-1 activity.

1. Dinarello, C.A. (1998) Int. Rev. Immunol. 16, 457-499.
2. Burns, K. et al. (1998) J. Biol. Chem. 273, 12203-12209.
3. Cao, Z. et al. (1996) Nature 383, 443-446.
4. Cao, Z. et al. (1996) Science 271, 1128-1131.
5. Wesche, H. et al. (1997) Immunity 7, 837-847.
6. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
7. Thornberry, N. A. et al. (1992) Nature 356, 768-774.
8. Cerretti, D. P. et al. (1992) Science 256, 97-100.
9. Grahames, C.B. et al. (1999) Br J Pharmacol 127, 1915-21.

• Basak, C. et al. (2005) J Biol Chem 280, 4279-88. Applications: Western Blotting

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For Research Use Only. Not For Use In Diagnostic Procedures.