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Hexokinase I Antibody

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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2025年10月30日
  • W
  • Rabbit
  • H,Mk,B
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体英文名

      Hexokinase I Antibody

    • 抗原

      synthetic peptide corresponding to the sequence of human hexokinase I

    • 应用范围

      W

    • 宿主

      Rabbit

    • 适应物种

      H,Mk,B

    • 供应商

      CST

    • 库存

      大量

    • 级别

      详见MSDS文件

    • 保质期

      详见说明书

    • 是否单克隆

      2

    • 保存条件

      -20°c

    • 规格

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting
    Reactivity Key:  H=Human  Mk=Monkey  B=Bovine
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Source
    W H Mk (B) Endogenous 102 Rabbit
    Protocols
    Specificity / Sensitivity

    Hexokinase I Antibody detects endogenous levels of total hexokinase I protein.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human hexokinase I. Antibodies are purified by peptide affinity chromatography.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from various cell types using Hexokinase I Antibody.

    Background

    Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

    1. Warburg, O. (1956) Science 123, 309-314.
    2. Semenza, G.L. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 71-103.
    3. Smith, T.A. (2000) Br. J. Biomed. Sci. 57, 170-178.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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    图标文献和实验
    相关实验
    • 体外荧光法检测核内体早期动力学

      that possess an intrinsic affinity toward each other (such as antigen and antibody, or avidin and biotin). After cell lysis, endosomes are allowed to fuse in vitro in the presence of an externally added quencher that prevents complex formation between markers

    • 投稿有关信件的写法

      only have been done and with exception of that summarized in Table 2, none has been repeated. This is clearly unsatisfactory, particularly when there is so much variation between assays. 10. The condition of incubation are poorly defined. What is the temperature? Were antibody used

    • 体外荧光法检测核内体早期动力学

      that possess an intrinsic affinity toward each other (such as antigen and antibody, or avidin and biotin). After cell lysis, endosomes are allowed to fuse in vitro in the presence of an externally added quencher that prevents complex formation between markers

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