- 询价
- Cell Signaling Technology
- 2448
- USA
- 2025年08月06日
- W, IP, IF-IC
- Rabbit
- All
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Glu-Glu Tag Antibody
- 抗原：
synthetic peptide
- 应用范围：
W, IP, IF-IC
- 宿主：
Rabbit
- 适应物种：
All
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | All | Transfected Only | N/A | Rabbit |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Glu-Glu Tag Antibody detects transfected proteins containing the Glu-Glu epitope tag. The antibody recognizes the Glu-Glu tag fused to either the amino- or carboxy-termini of targeted proteins in transfected mammalian cells. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of total cell lysates from Cos cells (lane 1), Cos cells expressing an N-terminal Glu-Glu-tagged protein (lane 2), and Cos cells expressing a C-terminal Glu-Glu-tagged protein (lane 3), using Glu-Glu Tag Antibody. IF-IC
Confocal immunofluorescent analysis of parental COS cells (left), COS cells expressing an N-terminal Glu-Glu-tagged protein (middle), and COS cells expressing a C-terminal Glu-Glu-tagged protein (right), labeled with Glu-Glu Tag Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Purification and Characterization of His-Tagged Antibody Fragments
, typically His6 , fused to the recombinant protein. In the case of antibody fragments that are composed of different chains, e.g., Fv and Fab, the site of attachment for the His6 tag has to be carefully chosen, and mild chromatographic conditions
Purification and Analysis of Strep-tagged Antibody-Fragments
The Strep-tag is a nine-residue minimal peptide sequence with intrinsic affinity towards streptavidin. This peptide – or its improved version Strep-tag II – can be fused to recombinant antibody fragments and other proteins in various fashions
Protein Antigen Expression in Escherichia coli for Antibody Production
Escherichia coli is a frequently used expression system for the generation of protein encoded by genes from diverse kingdoms and, thus, it is well suited for the production of protein antigens for antibody generation. It is a system of choice
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