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C/EBPalpha (p42) Antibody

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  • Cell Signaling Technology已认证
  • USA
  • 2025年08月06日
  • W
  • Rabbit
  • H,M,R
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    • 文献和实验
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    • 抗体英文名

      C/EBPalpha (p42) Antibody

    • 抗原

      synthetic peptide corresponding to the amino-terminal sequence of human C/EBPalpha

    • 应用范围

      W

    • 宿主

      Rabbit

    • 供应商

      CST

    • 级别

      详见MSDS文件

    • 适应物种

      H,M,R

    • 库存

      大量

    • 保质期

      详见说明书

    • 是否单克隆

      2

    • 保存条件

      -20°c

    • 规格

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting
    Reactivity Key:  H=Human  M=Mouse  R=Rat
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Source
    W H (M) (R) Endogenous 45 Rabbit
    Protocols
    Specificity / Sensitivity

    C/EBPalpha (p42) Antibody detects endogenous levels of total p42 C/EBPalpha protein but not p30 C/EBPalpha.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human C/EBPalpha. Antibodies are purified by protein A and peptide affinity chromatography.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from U937 cells, using C/EBPalpha (p42) Antibody.

    Background

    CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function, and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε, and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-I stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin-induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226, and Ser230 by GSK-3 seems to be required for adipogenesis (4).

    1. Lekstrom-Hims, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
    2. Lin, F. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 9606-9610.
    3. Hemati, N. et al. (1997) J. Biol. Chem. 272, 25913-25919.
    4. Ross, S.E. et al. (1999) Mol. Cell. Biol. 19, 8433-8441.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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    图标文献和实验
    相关实验
    • Antibody Conjugation

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • Antibody Conjugation Protocol

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • Antibody cleanup

      seven times, or as often as necessary to decrease the background. Pellet cells. Save antibody supernatant, which is now diluted 1:5 from its initial concentration. These antibodies can be stored at 4o C for several weeks or at -70o C for longer periods

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