BRF1/2 Antibody

2119:

Flow , Immunofluorescence , Western Blotting

This antibody detects endogenous levels of total BRF1 and BRF2 proteins.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human BRF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of cell lysates from HT1080, HT1080 BRF1^-/- , HCT15, C6, COS and NIH/3T3 cells, using BRF1/2 Antibody.

Flow Cytometry

Flow cytometric analysis of A549 cells, using BRF1/2 Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

Immunofluorescent analysis of DLD-1 cells, using BRF1/2 Antibody.

Butyrate response factor 1 (BRF1; also known as EGF response factor 1 [ERF1], TIS11B, ZFP36L1) and butyrate response factor 2 (BRF2; also known as EGF response factor 2 [ERF2], TIS11D, ZFP36L2) both belong to the TIS11 family of CCCH zinc-finger proteins (1). This family of proteins, which also includes tristetraprolin (TTP), bind to AU-rich elements (ARE) found in the 3'-untranslated regions of mRNAs and promote de-adenylation and rapid degradation by the exosome (2,3). These proteins play a critical role in cell growth control by regulating the mRNA turnover of multiple cytokines, growth factors and cell cycle regulators, including GM-CSF, TNFα, IL-2, IL-3 and IL-6 (4,5). Deregulated ARE-mRNA stability can contribute to both inflammation and oncogenic transformation (6-8). Insulin-induced stabilization of ARE-containing transcripts is mediated by Akt/PKB phosphorylation of BRF1 at Ser92, which results in binding by 14-3-3 protein and inactivation of BRF1 (9).

1. Varnum, B.C. et al. (1991) Mol. Cell. Biol. 11, 1754-1758.
2. Stoecklin, G. et al. (2002) EMBO J. 21, 4709-4718.
3. Lykke-Andersen, J. and Wagner, E. (2005) Genes Dev. 19, 351-361.
4. Stoecklin, G. et al. (2000) Mol. Cell. Biol. 20, 3753-3763.
5. Stoecklin, G. et al. (2001) RNA 7, 1578-1588.
6. Schuler, G.D. and Cole, M.D. (1988) Cell 55, 1115-1122.
7. Nair, A.P. et al. (1994) Nature 369, 239-242.
8. Carballo, E. et al. (1998) Science 281, 1001-1005.
9. Schmidlin, M. et al. (2004) EMBO J. 23, 4760-4769.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 9272 Akt Antibody
• 2966 Akt (5G3) Mouse mAb
• 2967 Akt1 (2H10) Mouse mAb
• 2964 Akt2 (5B5) Rabbit mAb
• 9916 Phospho-Akt Pathway Antibody Sampler Kit
• 9614 Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb
• 9636 14-3-3 β/α Antibody
• 9638 14-3-3 τ Antibody
• 9601 Phospho-(Ser) 14-3-3 Binding Motif Antibody
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.