- 询价
- Cell Signaling Technology
- 2119
- USA
- 2025年08月06日
- W, IF-IC, F
- Rabbit
- H,M,R,Mk,C,B
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
BRF1/2 Antibody
- 抗原：
synthetic peptide corresponding to the carboxy terminus of human BRF1 protein
- 应用范围：
W, IF-IC, F
- 宿主：
Rabbit
- 适应物种：
H,M,R,Mk,C,B
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC F | H M R Mk (C) (B) | Endogenous | 40 to 50, 62 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | This antibody detects endogenous levels of total BRF1 and BRF2 proteins. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human BRF1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of cell lysates from HT1080, HT1080 BRF1-/- , HCT15, C6, COS and NIH/3T3 cells, using BRF1/2 Antibody. Flow Cytometry
Flow cytometric analysis of A549 cells, using BRF1/2 Antibody (blue) compared to a nonspecific negative control antibody (red). IF-IC
Immunofluorescent analysis of DLD-1 cells, using BRF1/2 Antibody. |
| Background | Butyrate response factor 1 (BRF1; also known as EGF response factor 1 [ERF1], TIS11B, ZFP36L1) and butyrate response factor 2 (BRF2; also known as EGF response factor 2 [ERF2], TIS11D, ZFP36L2) both belong to the TIS11 family of CCCH zinc-finger proteins (1). This family of proteins, which also includes tristetraprolin (TTP), bind to AU-rich elements (ARE) found in the 3'-untranslated regions of mRNAs and promote de-adenylation and rapid degradation by the exosome (2,3). These proteins play a critical role in cell growth control by regulating the mRNA turnover of multiple cytokines, growth factors and cell cycle regulators, including GM-CSF, TNFα, IL-2, IL-3 and IL-6 (4,5). Deregulated ARE-mRNA stability can contribute to both inflammation and oncogenic transformation (6-8). Insulin-induced stabilization of ARE-containing transcripts is mediated by Akt/PKB phosphorylation of BRF1 at Ser92, which results in binding by 14-3-3 protein and inactivation of BRF1 (9).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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