- 询价
- Cell Signaling Technology
- 4323
- USA
- 2025年07月26日
- 流式细胞（Flow Cyt）
- 兔
- 人,小鼠,大鼠,驴
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- 详细信息
- 文献和实验
- 技术资料
- 级别：
详见MSDS文件
- 供应商：
CST
- 抗原：
/
- 抗体英文名：
Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb (Alexa Fluor 488 Conjugate)
- 是否单克隆：
1
- 库存：
大量
- 抗原来源：
/
- 保质期：
详见说明书
- 保存条件：
-20°c
- 适应物种：
人,小鼠,大鼠,驴
- 应用范围：
流式细胞（Flow Cyt）
- 宿主：
兔
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Jak/Stat Pathway
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4323
PhosphoSitePlus ® protein, site, and accession data: STAT3
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| F | H M R Mk | Endogenous | 86 kDa | Rabbit IgG |
Applications Key: F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4323:
- Flow
Specificity / Sensitivity
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or IFN-α treated (green), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4323 compared to nonspecific control antibody (red).
Description
This Cell Signaling Technology antibody conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody #9145 reacts with Phospho- Stat3 (Tyr705) from human, mouse and rat. CST expects that Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize Phospho-Stat3 in these species.
Background
The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
- Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
- Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
- Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
- Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
- Bromberg, J.F. et al. (1999) Cell 98, 295-303.
- Darnell, J.E. et al. (1994) Science 264, 1415-21.
- Ihle, J.N. (1995) Nature 377, 591-4.
- Wen, Z. et al. (1995) Cell 82, 241-50.
- Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
- Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
Application References
- Qu, P. et al. (2009) J Immunol 182, 1648-59. Applications: Flow Cytometry
- Anand, S. et al. (2011) Blood 118, 1610-21. Applications: Flow Cytometry
- Kalaitzidis, D. and Neel, B.G. (2008) PLoS One 3, e3776. Applications: Flow Cytometry
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 9145 Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb
- 4324 Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 9131 Phospho-Stat3 (Tyr705) Antibody
- 9138 Phospho-Stat3 (Tyr705) (3E2) Mouse mAb
- 9132 Stat3 Antibody
- 9139 Stat3 (124H6) Mouse mAb
- 2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
- 4340 Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
稀释液的效果更好。4. 匹配的二抗和显色系统——「配一配和滴一滴」使用多聚 HRP（Poly-HRP）的二抗，即在二抗上连接大量 HRP，以充分放大信号；同时，配合优质的色原产品，提升灵敏度，增强信号。注：在人肺癌标本中使用 Sox2(D6D9) XP® RabbitmAb#3579 检测 Sox2 的表达，使用基于多聚-HRP 的 SignalStain® BoostIHCDetectionReagent 的检测灵敏度更高。注：DAB 色原比较：在人乳腺癌组织中使用 p-Stat3(Tyr705)(D3A7
used in accordance withmanufacturer recommendationsSignalStain?DAB Substrate Kit #8059:IHC analysis of paraffin-embedded human breastcarcinoma using Phospho-Stat3 (Tyr705) (D3A7) XP? Rabbit mAb #9145. Chromogenicdetection was performed using #8059 (left
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