Applications Key: W=Western Blotting Reactivity Key: H=Human Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Snail (SN9H2) Rat mAb detects endogenous levels of total Snail.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant human Snail protein.
Western Blotting
Western blot analysis of total cell lysates from HCT116, 293 cells, and 293 cells transiently transfected with a SNAIL expressing construct, using Snail (SN 9H2) Rat mAb.
Background
Snail is a zinc-finger transcription factor that can repress E-cadherin transcription. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (1-3). Indeed, loss of E-cadherin expression is correlated with the invasive properties of some tumors and there is a considerable inverse correlation between Snail and E-cadherin mRNA levels in epithelial tumor cell lines (4,5). In addition, Snail blocks the cell cycle and confers resistance to cell death (6). Phosphorylation of Snail by GSK-3 and PAK1 regulates its stability, cellular localization and function (7-10).