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NUP98 (C39A3) Rabbit mAb

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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2025年07月15日
  • W, IP, IF-IC
  • H,M,R,Mk
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体英文名

      NUP98 (C39A3) Rabbit mAb

    • 抗原

      synthetic peptide corresponding to residues surrounding Pro671 of human NUP98

    • 应用范围

      W, IP, IF-IC

    • 保质期

      详见说明书

    • 级别

      详见MSDS文件

    • 库存

      大量

    • 适应物种

      H,M,R,Mk

    • 供应商

      CST

    • 是否单克隆

      1

    • 保存条件

      -20°c

    • 规格

      40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:40 ul (4 western blots)产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Isotype
    W IP IF-IC H M R Mk Endogenous 98 Rabbit IgG
    Protocols
    Specificity / Sensitivity

    NUP98 (C39A3) Rabbit mAb detects endogenous levels of total NUP98 protein.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro671 of human NUP98.

    Western Blotting

    Western Blotting

    Western blot analysis of cell extracts from various cell types using NUP98 (C39A3) Rabbit mAb.

    IF-IC

    IF-IC

    Confocal immunofluorescent analysis of Caki-1 cells using NUP98 (C39A3) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

    Background

    NUP98 (nucleoporin 98 kDa) is a component of the nuclear pore complex. It is expressed as three different precursors that undergo auto-cleavage to generate a common amino-terminal 98 kDa peptide (NUP98) and carboxy-terminal 6, 96 (NUP96) and 88 (p88) kDa peptides (1-2). NUP98 contains FG and GLFG repeat domains at its amino terminus and a RNA-binding domain in its carboxy terminus (3). The NUP98 gene is localized on chromosome 11p15.5, a region frequently rearranged in leukemias. To date, 15 fusion partners have been identified for NUP98 (4-5).

    1. Fontoura, B.M. et al. (1999) J. Cell Biol. 144, 1097-1112.
    2. Rosenblum, J.S. and Blobel, G. (1999) Proc. Natl. Acad. Sci. USA 96, 11370-11375.
    3. Griffis, E.R. et al. (2004) Mol. Biol. Cell 15, 1991-2002.
    4. Slape, C. and Aplan, P.D. (2004) Leuk. Lymphoma 45, 1341-1350.
    5. Nakamura, T. (2005) Int. J. Hematol. 82, 21-27.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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    图标文献和实验
    相关实验
    • Rabbit Whole Embryo Culture

      Although the rabbit is used extensively in developmental toxicity testing, relatively little is known about the fundamental developmental biology of this species let alone mechanisms underlying developmental toxicity. This paucity

    • Purification of mAb (IgG)

        Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers

    • Testing Rabbit Bleeds By Elisa

      as a solid if soluble. Mix, adding glutaraldehyde last. Peptide and BSA turn a little yellow even before adding glutaraldehyde. Incubate 90'' at 37 deg C. Add 0.1 volumes of 0.1 M NaBH4 in 0.1 M NaHCO3. There will be some bubbling. Add

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