- 询价
- Cell Signaling Technology
- 4996
- USA
- 2025年08月28日
- W, IHC-P, F
- Rabbit
- H,M,R
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- 详细信息
- 技术资料
- 抗体英文名：
UBC3B Antibody
- 抗原：
synthetic peptide corresponding to the sequence of human UBC3B
- 应用范围：
W, IHC-P, F
- 宿主：
Rabbit
- 适应物种：
H,M,R
- 库存：
大量
- 供应商：
CST
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P F | H M R | Endogenous | 32 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | UBC3B Antibody detects endogenous levels of total UBC3B protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human UBC3B. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from Jurkat and C6 cells using UBC3B Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using UBC3B Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Jurkat cells using UBC3B Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using UBC3B Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using UBC3B Antibody in the presence of control peptide (left) or antigen-specific peptide (right). Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells using UBC3B antibody (blue) compared to a nonspecific negative control antibody (red). |
| Background | Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). Recent data suggest that activated E2 associates transiently with E3, and that the dissociation is a critical step for ubiqitination (5). UBC3, the mammalian orthologue of yeast Cdc34, and UBC3B, a UBC3 family member, are E2 ubiquitin-carrier proteins. These proteins contain a conserved core domain containing a cysteine residue, which forms the thioester bond with ubiquitin (6). UBC3 in concert with the SCFSkp2 (Skp1, Cullin and F-box protein/Skp2) complex mediates cell cycle progression from G1 to S phase by targeting the CDK inhibitor p27 for proteolysis (7). UBC3B in concert with the SCFb-Trcp (Skp1, Cullin and F-box protein/b-Trcp) complex mediates degradation of b-catenin (6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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