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文献和实验Protein Array-Based Multiplexed Cytokine Assays
confocal slide scanner and quantitated using PerkinElmer QuantArray™ software. Experiments demonstrate that 43 cytokines can be simultaneously screened and quantitated in conditioned culture media, cell lysate, and human plasma. Using this chip
Human FastTrack mRNA Isolation
. Modified FastTrack 2.0 Protocol Isolation of mRNA 1.Add 15ml of Lysis Buffer (15ml Stock Buffer + 300 ul RNase/Protein Degrader) to the frozen pellet. 2.The tissue homogenizer should be cleaned so that it is RNase-free. At a minimum will require
Protocol for FastTrack 2.0 mRNA Extraction Human Cells
. Modified FastTrack 2.0 Protocol Isolation of mRNA Add 15ml of Lysis Buffer （15ml Stock Buffer 300 ul RNase/Protein Degrader） to the frozen pellet. The tissue homogenizer should be cleaned so that it is RNase-free. At a minimum will require immersion
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