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- Merck
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- 2025年11月14日
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文献和实验Xeno-Free Culture of Human Pluripotent Stem Cells
substratum: (1) TeSR2™ with human recombinant laminin (LN-511); (2) NutriStem™ with LN-511; (3) RegES™ with human foreskin fibroblasts (hFFs); (4) KO-SR Xeno-Free™/GF cocktail with CELLstart™ matrix.
CELL ADHESION Procedure: 1) Dissolve/dilute coating substrate in ddH2O at 4 C. A common working dilution for the laminin-1 positive control and BSA (Sigma A8412) negative control is 40 ug/ml. For SN-peptide, use 20 uM (MW of SN
Cell culture-based analysis of postsynaptic membrane assembly in muscle cells
has been their inability to recapitulate the multistage surface acetylcholine receptor (AChR) redistribution events that produce complex AChR clusters found at the intact neuromuscular junction (NMJ). By taking advantage of the ability of substrate laminin to induce
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