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文献和实验Small Scale Yeast Whole Cell Extract for IP
Grow 100 ml yeast cells in desired media overnight to an A600 of ~1.0 (0.6 to 1.2 works well). For growth in minimal media, 1 ml of a saturated overnight culture in minimal media (Synthetic dextrose (SD) with only the required amino acids
Purification of KHC Motor Domain Protein
Protease Inhibitor Cocktail (200X) = 1 microgram/mL Pepstatin A 1 microgram/mL Leupeptin 2 microgram/mL Aprotinin 2 microgram/mL TAME 1 M DTT 1 M MgCL
Purification of Ncd Motor Domain Protein
Cocktail (200X) = 1 microgram/mL Pepstatin A 1 microgram/mL Leupeptin 2 microgram/mL Aprotinin 2 microgram/mL TAME 1 M DTT 1 M MgCL2 10 mg
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