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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
Neobioscience
- CAS号:
26305-03-3
- 保存条件:
-20
- 规格:
1 x 5.0 x mg
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文献和实验Small Scale Yeast Whole Cell Extract for IP
Grow 100 ml yeast cells in desired media overnight to an A600 of ~1.0 (0.6 to 1.2 works well). For growth in minimal media, 1 ml of a saturated overnight culture in minimal media (Synthetic dextrose (SD) with only the required amino acids
核蛋白基因组指纹技术Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
temperature; add PMSF and proteinase inhibitors and put on ice before use) 5 mM PIPES (pH 8) 85 mM KCl 0.5% NP-40 1 mM PMSF proteinase inhibitors (leupeptin, aprotinin, pepstatin; each final concentration 2 µg/ml) Nuclear
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