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文献和实验Purification of Calmodulin from Bovine Brain
. Procedure (0-4℃): 1.About 1 kg of bovine brain (PelFreeze,frozen)is homogenized in 2 volumes of homogenizing buffer (50 mM MOPS,pH 7.0,1.0 mM 2-ME,1.0 mM EDTA)for 90 sec in a large Waring blender. 2.The homogenate is centrifuged at 10,000 x g for 1 hr
Adult cattle serum as supplemental nutrient in lymphocyte cultures for chromosomes studies
: Mon Feb 02 2009 Date Modified: Mon Feb 02 2009 Abstract: The author has made a search for an alternate nutrient (serum supplement) source for lymphocyte cultures. This report describes the procedure for separation
Procedures for In Vitro Production of Bovine Embryos
manufacturer’s indications for expiration date. Store at 4°C.Stock 2: Na pyruvate. Dissolve 0.220 g sodium pyruvate in 100 ml water. Sterile-filter into an aluminum-foil wrapped 100 ml bottle and store at 4°C for 1 mo.Stock 3: Bovine Steer Serum (BSS
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