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文献和实验Pronase is the name given to a group of proteolytic enzymes that are produced in the culture supernatant of Streptomyces griseus K-1 (1 –3 ). Pronase is known to contain at least ten proteolytic components: five serine-type proteases, two Zn
Streptomyces:Protocols/Conjugation
Intergeneric Conjugation and Overlay Description Transfer of plasmid/cosmid DNA from a host strain, e.g. E.coli ET12567 [pUZ8002], to the recipient strain, e.g. Streptomyces coelicolor. Approx. Duration
of target DNA thus determining the beginning and end of the region to be amplified, e.g. flanking a gene on genomic DNA. The DNA polymerase uses Deoxynucleotide-triphosphates (dNTPs), a mix of dATP, dCTP, dGTP & dTTP in its synthesis of the complementary
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