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- Merck
- 美国
- 05-554
- 2025年07月11日
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文献和实验Single Clone Excision Protocol
500ml 20% maltose and 500ml 1 M MgSO4 to 50 ml LB broth). Next day: 3.If you're making new cells,spin them down 5 min at 1500 x g in the C0650 rotor in the Beckman centrifuge,then resuspend in about 40 ml 10 mM MgSO4,and quantify absorbance
Construction and Manipulation of Large-Insert Bacterial Clone Libraries
Introduction Construction of Large-Insert Bacterial Clone Libraries Fig. 1. A general procedure
Construction of cDNA Libraries in gt10 or gt11
that it is possible to divide the mRNA population into three classes with most of the mRNAs (11,300) being in the lowest abundance class and making up 39% of the polysomal mRNA. To obtain a cDNA library that contains at least one clone for each mRNA of this class
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