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文献和实验Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification
Protein purification is an important tool for investigations on protein function, structure analysis, and biotechnological use. Therefore a number of different techniques have been developed for fast, reliable, and reproducible overexpression
Characterization of Calcium/Calmodulin- Stimulated Protein Kinase II
The enzyme Ca2+ /calmodulin-stimulated protein kinase II (CaM-PK II) belongs to a family of calmodulin-stimulated serine/threonine protein kinases that are widely distributed in nature, but are especially enriched in the brain (1 –3 ). CaM-PK
Assay and Purification of Calmodulin-Dependent Protein Kinase
Posttranslation covalent modification of proteins by phosphorylation represents an important mechanism in the control of many cellular functions (1 ,2 ). Protein kinases catalyze the transfer of the γ-phosphoryl group of ATP to an acceptor
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